Methods and compositions for galactosylated glycoproteins

Galactosylierte glykoproteine enthaltende zusammensetzungen und verfahren zur deren herstellung

Procedes et compositions concernant des glycoproteines galactosylees

Abstract

This invention relates to novel glycoprotein glycoform preparations comprising the substantially homogenous glycoprotein glycoforms. More particularly the invention relates to substantially homogenous glycoprotein preparations comprising a particular Fc glycan and methods for producing, detecting, enriching and purifying the glycoforms. The invention further relates to immunoglobulins and especially antibodies comprising a CH2 domain having a particular glycan. Provided are compositions including pharmaceutical compositions, methods of using the preparations as well as articles of manufacture comprising the preparations.
L'invention a trait à de nouvelles préparations de glycoformes glycoprotéiques contenant les glycoformes glycoprotéiques sensiblement homogènes. L'invention concerne plus particulièrement des préparations de glycoprotéines sensiblement homogènes renfermant un glycanne Fc, ainsi que des procédés permettant de fabriquer, déceler, enrichir et purifier les glycoformes. En outre, l'invention se rapporte à des immunoglobulines et, en particulier, à des anticorps renfermant un domaine CH2 contenant un glycanne particulier. L'invention concerne enfin des compositions, notamment des compositions pharmaceutiques, des procédés d'utilisation de ces préparations ainsi que des produits manufacturés renfermant ces préparations.

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